Journal: Archives of Microbiology

Article Title: Methanobrevibacter smithii activates immune microenvironment of intestinum tenue in a mouse model

doi: 10.1007/s00203-026-04772-2

Figure Lengend Snippet: Cytokine changes in colon after M. smithii gavage in mouse model. A Western blots showed expressions of TNF-ɑ, IL-22, IL-17 and GM-CSF in colon tissues collected from the control and experimental groups. B-E Quantifications of the relative gray value of western blots analyzed by ImageJ software. F Representative images of expressions of TNF-ɑ, IL-22, IL-17 and GM-CSF in colon sections of PBS and M.smithii groups. G-J Quantifications of staining density of cytokines of TNF-ɑ, IL-22, IL-17 and GM-CSF in colon sections of PBS and M.smithii groups. K-N RNA expression levels of TNF-ɑ, IL-22, IL-17 and GM-CSF in colon of the two groups were analyzed by RT-qPCR. Data are shown as mean ± SEM, two-tailed Student’s t-test was used to perform statistical comparisons between groups, n = 8 in each independent experiment, scale bar: 50 μm, each experiment was repeated three times

Article Snippet: Membranes were blocked with 5% non-fat dry milk in Tris-buffered saline containing 0.5% Tween-20 (TBST) for 1 h at room temperature, then probed overnight at 4 °C with the following primary antibodies (1:2000 dilution): TNF-α (ab215188), IL-17 (13838 S), GM-CSF (505504), and IL-22 (A6212) from respective suppliers (Abcam; CST; BioLegend; ABclonal).

Techniques: Western Blot, Control, Software, Staining, RNA Expression, Quantitative RT-PCR, Two Tailed Test

Journal: Archives of Microbiology

Article Title: Methanobrevibacter smithii activates immune microenvironment of intestinum tenue in a mouse model

doi: 10.1007/s00203-026-04772-2

Figure Lengend Snippet: ILC3s can be activated by M.smithii stimuli. A Representative flow cytometry plots showing the populations of ILCs. B Representative flow cytometry plots of ILC3s cells. C Statistical comparisons of CD117 + cells in the PBS and M.smithii groups. D Representative flow cytometry plots of CD117 + TNF-ɑ+ cells. E Statistical comparisons of CD117 + TNF-ɑ+ percentages in the PBS and M.smithii groups. F Representative flow cytometry plots of CD117 + IL-17 + cells. G. Statistical comparisons of the proportions of CD117 + IL-17 + cells in the groups of PBS and M.smithii . H Representative flow cytometry plots of CD117 + IL-22 + cells. I Statistical comparisons of CD117 + IL-22 + cells proportions in PBS and M.smithii groups. J Representative flow cytometry plots of CD117 + GM-CSF+ cells. K Statistical comparisons of CD117 + GM-CSF+ cells in the PBS and M.smithii groups. Data are shown as mean ± SEM, two-tailed Student’s t-test was used to perform statistical comparisons between groups, n = 8 in each independent experiment, each experiment was repeated three times

Article Snippet: Membranes were blocked with 5% non-fat dry milk in Tris-buffered saline containing 0.5% Tween-20 (TBST) for 1 h at room temperature, then probed overnight at 4 °C with the following primary antibodies (1:2000 dilution): TNF-α (ab215188), IL-17 (13838 S), GM-CSF (505504), and IL-22 (A6212) from respective suppliers (Abcam; CST; BioLegend; ABclonal).

Techniques: Flow Cytometry, Two Tailed Test

Journal: Archives of Microbiology

Article Title: Methanobrevibacter smithii activates immune microenvironment of intestinum tenue in a mouse model

doi: 10.1007/s00203-026-04772-2

Figure Lengend Snippet: Cytokine secretion alterations of CD4 + T cells after M.smithii gavage stimulation. A Representative flow cytometry plots of CD4 + T and CD8 + T cells in colon lamina propria cells. B , C Statistical comparisons of CD4 + T and CD8 + T cells of colon lamina propria in the PBS and M.smithii mice. D Comparisons of CD4 + T/ CD8 + T ratio in PBS and M.smithii groups. E , F Representative flow cytometry plots of CD4 + TNF-ɑ+cells, and statistical comparisons of CD4 + TNF-ɑ+cells in the PBS and M.smithii groups. G , H Representative flow cytometry plots of CD4 + IL-22 + cells, and statistical comparisons of CD4 + IL-22 + cells in the groups of PBS and M.smithii groups. I , J Representative flow cytometry plots of CD4 + IL-17 + cells, and statistical comparisons of CD4 + IL-17 + cells in the groups of PBS and M.smithii groups. K , L Representative flow cytometry plots of CD4 + GM-CSF+ cells, and statistical comparisons of CD4 + GM-CSF+ cells in the groups of PBS and M.smithii . Data are shown as mean ± SEM, two-tailed Student’s t-test was used to perform statistical comparisons between groups, n = 8 in each independent experiment, each experiment was repeated three times

Article Snippet: Membranes were blocked with 5% non-fat dry milk in Tris-buffered saline containing 0.5% Tween-20 (TBST) for 1 h at room temperature, then probed overnight at 4 °C with the following primary antibodies (1:2000 dilution): TNF-α (ab215188), IL-17 (13838 S), GM-CSF (505504), and IL-22 (A6212) from respective suppliers (Abcam; CST; BioLegend; ABclonal).

Techniques: Flow Cytometry, Two Tailed Test

Journal: Archives of Microbiology

Article Title: Methanobrevibacter smithii activates immune microenvironment of intestinum tenue in a mouse model

doi: 10.1007/s00203-026-04772-2

Figure Lengend Snippet: Effect of M.smithii stimuli on CD8 + T cells. A , B Representative flow cytometry plots of CD8 + TNF-ɑ+ cells, and statistical comparisons of CD8 + TNF-ɑ+ cells in the PBS and M.smithii groups. C , D Representative flow cytometry plots of CD8 + IL-22 + cells, and statistical comparisons of CD8 + IL-22 + cells in the groups of PBS and M.smithii . E , F Representative flow cytometry plots of CD8 + IL-17 + cells, and statistical comparisons of CD8 + IL-17 + cells in the groups of PBS and M.smithii . G , H Representative flow cytometry plots of CD8 + GM-CSF+ cells, and statistical comparisons of CD8 + GM-CSF+ cells in the groups of PBS and M.smithii . Data are shown as mean ± SEM, two-tailed Student’s t-test was used to perform statistical comparisons between groups, n = 8 in each independent experiment, each experiment was repeated three times

Article Snippet: Membranes were blocked with 5% non-fat dry milk in Tris-buffered saline containing 0.5% Tween-20 (TBST) for 1 h at room temperature, then probed overnight at 4 °C with the following primary antibodies (1:2000 dilution): TNF-α (ab215188), IL-17 (13838 S), GM-CSF (505504), and IL-22 (A6212) from respective suppliers (Abcam; CST; BioLegend; ABclonal).

Techniques: Flow Cytometry, Two Tailed Test